From: Adam Gaertner
Date: March 11, 2020 at 6:16 AM EDT
To: Fauci, Anthony (NIH/NIAID)
Subject: Coronavirus bioweapon production method
Hello Anthony,
This is how the virus was created.
Intervirion Fusion. HIV-luc(ACE2)(500 ng of p24) was mixed with 1,000 ng of p24 of HIV-gfp particles incorporating ASLV-A envelope, SARS-CoV S protein, or both envelopes in PBS at 4°C for 30 min to allow binding. Virions were adjusted to the desired pH with 0.1 M citric acid. PBS, TPCK-trypsin (final concentration 10 ng/ml), CTSL, cathespin B (CTSB) (final concentrations 2 ng/ml) or CTSL buffer alone was then added. Recombinant CTSL (R&D Systems) was preactivated by incubation for 15 min at 10 ng/ml in 50 mM Mes, pH 6.0, on ice. Recombinant CTSB (R&D Systems) was preactivated in 25 mM Mes, 5mM DTT, pH 5.0, for 30 min at 25°C. After a 10-min incubation at 25°C, proteolysis was halted by the addition of 300 nl of DMEM10 containing leupeptin (25 ng/ml) and STI (75ng/ml). Virions were then incubated at 37°C for 30 min to allow membrane fusion. 100 nl of the virion mixture was added in quadruplicate to HeLa-Tva cells pretrated for 1 h with leupeptin (20 ng/ml). The cells were spin-infected and incubated at 37°C for 5 h.
There is more at Karl Denninger's.
UPDATE: The acceptance of the not-vaxx appears to be much lower than the media is reporting. It may be as low as 30 percent. This would explain the increasing desperation of the pushers and the ridiculous "incentives" being offered to take the death-marker.
http://voxday.blogspot.com/
